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Updated: Feb 11, 2026

Proteomic Profiling of Macrophages by 2D Electrophoresis
Published on: November 4, 2014
Proteomic profiling of Ganoderma tsugae ethanol extract-induced adipogenesis displaying browning features
Hsiu-Hsueh Tseng1, Wan-Chun Yeh2, Yun-Chen Tu2
1Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC.
Abstract:
Ganoderma is classified as a top grade traditional Chinese medicine for promoting human health by regulating 'vital energy'. Its potency towards metabolism and energy homeostasis, particularly, metabolic adaptations of adipocytes, needs to be re-evaluated through an evidence-based study. Here, the triterpenoid-rich Ganoderma tsugae ethanol extract (GTEE) was found to contribute towards adipogenesis accompanied with elevated intracellular lipid metabolic flux. Additionally, proteomic profiling revealed GTEE-upregulated mitochondrial remodeling and chemical energy redox modifications, which display UCP1-positive browning fat-selective features and a NADH-mediated adaptive mechanism. GTEE-treated mice with diet-induced obesity also resulted in the amelioration of white adipocyte hypertrophy and the appearance of UCP1-positive browning adipocytes. Our novel findings unravel that GTEE could promote intracellular metabolic flexibility and plasticity followed by the induction of adipocyte browning.
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