Efficient Capture and High Activity Release of Circulating Tumor Cells by Using TiO₂ Nanorod Arrays Coated with...

Effective capture and release of circulating tumor cells (CTCs) with high viability is still a challenge in medical research. We design a novel approach with efficient yield and high cell activity for the capture and release of CTCs. Our platform is based on TiO₂ nanorod arrays coated with transparent MnO₂ nanoparticles. We use hydrothermal synthesis to prepare TiO₂ nanorod arrays, the MnO₂ nanoparticles are fabricated through in situ self-assembly on the substrate to form a monolayer and etched by oxalic acid with low concentration at room temperature. Up to 92.9% of target cells are isolated from the samples using our capture system and the captured cells can be released from the platform, the saturated release efficiency is 89.9%. Employing lower than 2 × 10^-3 M concentration of oxalic acid to dissolve MnO₂, the viability of MCF-7 cancer cells exceed 90%. Such a combination of the two-dimensional and three-dimensional platforms provides a new approach isolate CTCs from patient blood samples.