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Draft genome sequence of field isolate Brucella melitensis strain 2007BM/1 from India

D K Singh1, Bablu Kumar2, Garima Shrinet2

  • 1Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India.

Journal of Global Antimicrobial Resistance
|April 24, 2018
PubMed
Abstract

Insights

This study presents the draft genome sequence of Brucella melitensis, a significant zoonotic pathogen endemic in India. Understanding its drug resistance and virulence factors is crucial for public health and livestock management.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Brucellosis is a widespread zoonotic disease globally, particularly prevalent in India.
  • Brucella melitensis is recognized as the most pathogenic species for human infections.

Purpose of the Study:

  • To report the draft genome sequence of Brucella melitensis strain 2007BM/1, isolated from a human case in India.
  • To identify potential drug resistance and virulence factors within this strain.

Main Methods:

  • Genomic DNA extraction from Brucella culture.
  • Whole-genome sequencing using Illumina MiSeq platform.
  • De novo assembly and annotation of the draft genome.

Main Results:

  • Sequenced a monoisolate with a genome length of 3,268,756 bp.
  • Identified resistance to azithromycin and trimethoprim/sulfamethoxazole.
  • Confirmed susceptibility to tetracycline, ofloxacin, rifampicin, ciprofloxacin, and doxycycline.
  • Detected the presence of virulence genes.

Conclusions:

  • The findings aid in understanding drug resistance mechanisms and virulence factors in B. melitensis.
  • Highlights the need for careful antibiotic use in livestock to manage this highly zoonotic bacterium.
Keywords:
Antibiotic susceptibilityBrucellaGenome sequencingVirulence factors

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