Size-dependent endocytosis and a dynamic-release model of nanoparticles

Polymeric nanoparticles for drug delivery are attracting broad interest along with the rapid development of biomedical and healthcare research. Here, we prepared a series of nanocapsules via electrostatic precipitation of chitosan and lecithin micelles. These nanocapsules have controlled diameters (∼25-200 nm) that only slightly changed after several lyophilization-dissolving cycles, suggesting their excellent stability for long-term storage. In cell experiments, these nanocapsules obviously reduced the cytotoxicity of encapsulated small molecules, and clearly showed size-dependent endocytosis. In a dynamic release model mimicking the in vivo circulatory system, the nanocapsules demonstrated superiority over micelles as drug carriers due to their stable structures. To the best of our knowledge, this is the first dynamic model used to evaluate the drug-release behaviour, which might provide a new way to study the release profile of other potential drug carriers.