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Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells

Anthony D Foster1,2, Nicholas Clark1, Thomas A Davis3,4

  • 1Regenerative Medicine Department, Naval Medical Research Center, Silver Spring, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 25, 2018
PubMed

Murine models of allograft transplantation are valuable for understanding the immunological mechanisms of allograft acceptance and rejection, the evaluation of immunosuppressive drugs and strategies, and the restoration of functional defects. Herein, we describe methods to create a skin murine allograft surgical model and how to administer adipose-derived stromal cells (ASC) with limited numbers of donor bone marrow to create stable multilineage donor cell chimerism and indefinite immunological tolerance.

Keywords:
Adipose stem cellsDonor cell chimerismIn vivo immune regulationSkin allograft transplantationTolerance induction

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