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Published on: October 27, 2023
High Definition Confocal Imaging Modalities for the Characterization of Tissue-Engineered Substitutes
Dominique Mayrand1,2, Julie Fradette3,4,5
1Centre de Recherche en Organogénèse Expérimentale de l'Université Laval/LOEX, CRCHU de Québec-Université Laval, Québec, QC, Canada.
Abstract:
Optimal imaging methods are necessary in order to perform a detailed characterization of thick tissue samples from either native or engineered tissues. Tissue-engineered substitutes are featuring increasing complexity including multiple cell types and capillary-like networks. Therefore, technical approaches allowing the visualization of the inner structural organization and cellular composition of tissues are needed. This chapter describes an optical clearing technique which facilitates the detailed characterization of whole-mount samples from skin and adipose tissues (ex vivo tissues and in vitro tissue-engineered substitutes) when combined with spectral confocal microscopy and quantitative analysis on image renderings.
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