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Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
Published on: September 26, 2013
Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates
Jie Li1,2, J Lowry Curley1,2, Z Elizabeth Floyd3
1Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA.
Abstract:
Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large scale procedure suitable for processing >100 mL volumes of lipoaspirate tissue specimens by collagenase digestion and a related procedure suitable for processing adipose tissue aspirates without digestion.
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