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Dynamic and Cell-Specific DACH1 Expression in Human Neocortical and Striatal Development.
Valentina Castiglioni1, Andrea Faedo1,2, Marco Onorati1,3,4
1Department of Biosciences, Istituto Nazionale di Genetica Molecolare, University of Milan and INGM, Milan, Italy.
Cerebral Cortex (New York, N.Y. : 1991)
|April 25, 2018
Summary
DACH1 expression is detailed in human neurodevelopment, particularly in developing cortical regions and the striatum. This study identifies DACH1 as a novel marker for striatal projection neurons.
Area of Science:
- Developmental neuroscience
- Human embryology
- Gene expression analysis
Background:
- DACH1, a homolog of the Drosophila dachshund gene, plays roles in development.
- Understanding DACH1's role in human neurodevelopment is crucial.
Purpose of the Study:
- To systematically investigate DACH1 expression patterns during human neurodevelopment.
- To identify novel markers for specific neuronal populations.
Main Methods:
- Immunodetection analysis of DACH1 expression.
- Single-cell global transcriptional analysis.
- Comparative analysis of DACH1 expression in human and mouse forebrain.
Main Results:
- DACH1 is highly expressed in proliferating neuroprogenitors in the developing human cortex.
- DACH1 is enriched in neuroepithelial and radial glia cells of the neocortex.
- DACH1 is newly identified in human striatal medium spiny neurons, serving as a potential marker.
Conclusions:
- DACH1 plays a significant role in early human cortical development.
- DACH1 is a novel marker for human striatal projection neurons.
- Spatio-temporal expression patterns of DACH1 show similarities between human and mouse forebrain development.
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