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Published on: April 25, 2014
Integrated analysis of methylome, transcriptome and miRNAome of three pig breeds
Yuhua Fu1, Jingxuan Li1, Qianzi Tang2
1Key Lab of Agriculture Animal Genetics, Breeding, & Reproduction of Ministry of Education, College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
Aim:
Integrated analysis of methylome and transcriptome may help understand the molecular basis of the different breeds with different traits of commercial interest.
Materials & Methods:
We obtained the first genome-wide methylome with single-base resolution, miRNAome and transcriptome from three swine breeds.
Results:
We displayed the landscape of the three omics in the whole-genome level. Integrated outcomes of methylome with genetic selection, miRNAome and transcriptome are also provided. Finally, we identified 11 candidate differentially methylated genes associated with phenotype variance in pigs.
Conclusion:
DNA methylation not only suppresses transcriptome but also miRNAome. The different -omics data have complicated interaction in directly or indirectly and exhibited close relations with the distinct phenotypic traits of growth, disease resistance and energy metabolism.
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