Related Experiment Video

Updated: Feb 11, 2026

Dry Root Rot Disease Assays in Chickpea: a Detailed Methodology
06:58

Dry Root Rot Disease Assays in Chickpea: a Detailed Methodology

Published on: January 17, 2021

8.2K

Antifungal Compound Isolated from Catharanthus roseus L. (Pink) for Biological Control of Root Rot Rubber Diseases

R Zahari1, N Halimoon2, M F Ahmad3

  • 1Laboratory of Sustainable Bioresource Management (BIOREM), Institute of Tropical Forestry and Forest Products (INTROP), Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

International Journal of Analytical Chemistry
|April 26, 2018
PubMed

Insights

Catharanthus roseus extract shows promise in controlling rubber root rot fungi. The CRD4 extract, containing ursolic acid, effectively inhibited Rigidoporus microporus and Ganoderma philippii, suggesting potential as a biocontrol agent.

Area of Science:

  • Agricultural Science
  • Mycology
  • Plant Chemistry

Background:

  • Root rot diseases caused by fungi like Rigidoporus microporus, Ganoderma philippii, and Phellinus noxius pose significant threats to rubber plantations.
  • Environmentally safe, plant-derived compounds are sought for sustainable disease management.

Purpose of the Study:

  • To investigate the antifungal efficacy of compounds isolated from Catharanthus roseus against key rubber root rot fungi.
  • To identify the specific compound responsible for antifungal activity and assess its potential as a biocontrol agent.

Main Methods:

  • Extraction of antifungal compounds from Catharanthus roseus using dichloromethane (DCM).
  • Thin Layer Chromatography (TLC) for separation and visualization of compounds.
  • Nuclear Magnetic Resonance (NMR) spectroscopy for structural identification.
  • In vitro screening of extracts against Rigidoporus microporus, Ganoderma philippii, and Phellinus noxius.

Main Results:

  • Catharanthus roseus extracts showed varying levels of antifungal activity.
  • The CRD4 extract demonstrated the highest efficacy against Rigidoporus microporus (3.5 mm inhibition zone) and Ganoderma philippii (1.9 mm inhibition zone).
  • NMR analysis identified ursolic acid in the CRD4 extract; however, none of the extracts were effective against Phellinus noxius.

Conclusions:

  • Ursolic acid from Catharanthus roseus exhibits potential as a biocontrol agent against Rigidoporus microporus and Ganoderma philippii.
  • Further research is warranted to explore the full potential of Catharanthus roseus extracts in managing rubber root rot diseases, particularly against Phellinus noxius.

More Related Videos

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds08:54

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds

Published on: February 14, 2018

20.6K
Measuring Volatile and Non-volatile Antifungal Activity of Biocontrol Products06:47

Measuring Volatile and Non-volatile Antifungal Activity of Biocontrol Products

Published on: December 5, 2020

7.4K

Related Concept Videos

Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
907
Molecules and Compounds02:38

Molecules and Compounds

Atoms and Molecules
69.4K
Organic Compounds03:02

Organic Compounds

All living things are formed mostly of carbon compounds called organic compounds. The category of organic compounds includes both natural and synthetic compounds that contain carbon. Although a single, precise definition has yet to be identified by the chemistry community, most agree that a defining trait of organic molecules is the presence of carbon as the principal element, bonded to hydrogen and other carbon atoms. However, some carbon-containing compounds such as carbonates, cyanides, and...
57.6K
What is Conservation Biology?01:57

What is Conservation Biology?

Conservation biology is a scientific field that focuses on the preservation of biodiversity in order to protect ecosystems while meeting the needs of the human population. Humans require properly functioning ecosystems to maintain our supply of natural resources, including food, medicines, and building materials.
24.4K
Elements and Compounds01:27

Elements and Compounds

Pure substances consist of only one type of matter. A pure substance can be an element or a compound. An element consists of only one type of atom, while a compound consists of two or more types of atoms held together by a chemical bond.
Elements
Elements are classified as atomic or molecular based on the nature of their basic units. They are unique forms of matter with specific chemical and physical properties that cannot break down into smaller substances by ordinary chemical reactions. There...
105.3K
Primary and Secondary Growth in Roots and Shoots03:02

Primary and Secondary Growth in Roots and Shoots

Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
60.7K