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"Dip-and-read" paper-based analytical devices using distance-based detection with color screening.

Kentaro Yamada1, Daniel Citterio, Charles S Henry

  • 1Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, USA. chuck.henry@colostate.edu.

Lab on a Chip
|April 26, 2018
PubMed
Summary

This study introduces a faster paper-based analytical device (PAD) using color screening for quick quantification. This innovative method significantly reduces assay time for detecting analytes like nickel in welding fume.

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Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • Paper-based analytical devices (PADs) offer portable and cost-effective analysis.
  • Traditional distance-based PADs face limitations in assay speed due to capillary flow rates.
  • Current methods can require up to 45 minutes for analysis over a 5 cm channel.

Purpose of the Study:

  • To develop an improved paper-based analytical device (PAD) utilizing color screening for enhanced performance.
  • To enable rapid, quantitative, and straightforward assays using a "dip-and-read" approach.
  • To overcome the time limitations of traditional distance-based detection motifs in PADs.

Main Methods:

  • Implemented a color screening method for PADs, converting color intensity to a distance-based signal.
  • Utilized inkjet-printing to deposit a colorimetric indicator line onto a paper substrate.

Related Experiment Videos

  • Developed a color filter with a continuous gradient to match the indicator line's color intensity.
  • Performed nickel quantification in welding fume as a model assay.

    • Main Results:

    • Achieved quantitative results in minutes (approximately 10 minutes) using the "dip-and-read" color screening method.
    • Demonstrated improved accuracy compared to visual color comparison: mean absolute percentage error of 10.5% and average relative standard deviation of 11.2%.
    • Confirmed practical utility through interference studies and shelf-life investigations.

    Conclusions:

    • The developed color screening approach provides a fast, quantitative, and straightforward alternative detection method for distance-based PADs.
    • This innovation significantly reduces assay times, making PADs more efficient for various analytical applications.
    • The system enhances the practical utility and performance of paper-based analytical devices.