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An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by

Sayuri Hara-Kuge1,2, Tomonobu Nishihara1, Tomoki Matsuda3

  • 1Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.

Plos One
|April 26, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed inverse-pericam 2.0 (IP2.0), a novel genetically encoded Ca2+ indicator. IP2.0 sensitively detects neuronal inhibition by monitoring decreased intracellular Ca2+ levels, advancing neuronal activity analysis.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Neuronal circuit function relies on coordinated excitation and inhibition.
  • Genetically encoded Ca2+ indicators (GECIs) monitor neuronal excitation via Ca2+ increases.
  • Current GECIs are unsuitable for sensitively detecting neuronal inhibition.

Purpose of the Study:

  • To develop a novel GECI capable of sensitively detecting neuronal inhibition.
  • To overcome the limitations of existing GECIs in monitoring inhibitory neuronal activity.

Main Methods:

  • Development of inverse-pericam 2.0 (IP2.0), an inverse-type GECI.
  • In vitro characterization of IP2.0's Ca2+ binding properties (25-fold fluorescence decrease).
  • In vivo application of IP2.0 to monitor Ca2+ dynamics in Caenorhabditis elegans neurons.

Main Results:

  • IP2.0 demonstrated a significant fluorescence decrease upon Ca2+ binding.
  • Successfully detected putative neuronal inhibition in AWCON and ASEL neurons.
  • Monitored decreases in intracellular Ca2+ concentration indicative of neuronal inhibition.

Conclusions:

  • IP2.0 is a valuable tool for studying neuronal inhibition.
  • Enables sensitive detection of reduced intracellular Ca2+ concentrations in vivo.
  • Facilitates detailed analysis of neuronal activities, including inhibition.