Effect of long-term caffeine administration to mice on in vitro fertilization and embryo development using oocytes
Hidemi Yokota1, Yoshimasa Yokota1, Mikako Yokota1
1Yokota Maternity Hospital 1-5-22 Shimokoide 371-0031 Maebashi Gunma Japan.
Purpose:
To evaluate the effect of long-term caffeine administration to mice on in vitro fertilization (IVF) of oocytes.
Methods:
Mice were injected with different dosages (0, 0.1, and 1.0 mg/mouse/converted day) of caffeine for one month. Subsequently, the fertilization rate and embryo development to blastocyst stage were evaluated in IVF using oocytes from the mice.
Results:
The retrieved average oocyte rate was significantly lower (27.4) in mice injected with 1.0 mg caffeine than in the control group (36.5; P < 0.05); the fertilization rate was significantly different between the 0 mg (317/401; 79.1 %) and 1.0 mg group (199/301; 66.1 %) (P < 0.05). At 96 h after insemination, the blastocyst formation rate was significantly decreased in the 1.0 mg group (94/199; 47.2 %) compared with the control (0 mg) group (237/317; 74.8 %) and 0.1 mg group (226/323; 70 %) (P < 0.05). When 1.0 mg caffeine was administered for two weeks, embryo development was significantly impacted.
Conclusions:
Our findings suggest that caffeine administration negatively impacts oocytogenesis and embryonic development after IVF.
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