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Effects of TRIS and HEPES on function of rabbit muscle light sarcoplasmic reticulum
B S Selinsky1, A D Messana, W Scherer
1Department of Biochemistry, School of Medicine, University of Buffalo, SUNY 14214.
Membrane Biochemistry
|January 1, 1987
Abstract:
ATP hydrolysis activity and calcium transport activity were determined on light sarcoplasmic reticulum from rabbit skeletal muscle. The effects of two buffers, TRIS and HEPES, were compared. Titration of TRIS into sarcoplasmic reticulum preparations in HEPES provided evidence for TRIS inhibition of ATPase activity and TRIS stimulation of calcium transport activity.