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Platelet-rich plasma as an additional therapeutic option for infected wounds with multi-drug resistant bacteria: in
Rıza Aytaç Çetinkaya1, Ercan Yenilmez1, Patrizio Petrone2,3
1Department of Infectious Disease, Sultan Abdulhamid Han Training and Education Hospital, University of Health Science, Istanbul, Turkey.
Purpose:
Infected wounds, such as diabetic foot infections, are mostly polymicrobial and microorganisms have high resistance rates to antimicrobials. Infected wounds in diabetic patients have high cost, morbidity, and mortality rates. Based on these facts, there is a need for supportive localized treatment options such as platelet-rich plasma (PRP) implementations. Demonstrating the in vitro antimicrobial effect, our aim was to lead up to clinical trials of localized PRP implementations in infected wounds such as diabetic foot infections. In this study, we aimed to demonstrate the in vitro antibacterial activity of PRP against methicilin-resistant Staphylococcus aureus (MRSA) and three more multi-drug resistant bacteria species that are important and hard-to-treat in wound infections.
Materials And Methods:
In vitro antimicrobial activity of autologous PRP, platelet-poor plasma (PPP), and phosphate-buffered saline (PBS) on methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus spp., extended spectrum beta lactamase producing Klebsiella pneumoniae, and carbapenem-resistant Pseudomonas aeruginosa was compared by assessment of bacterial growth on agar plates and antimicrobial susceptibility test results.
Results:
When compared to control group, PRP and PPP significantly suppressed bacterial growth of MRSA, K. pneumoniae, and P. aeruginosa at 1st, 2nd, 5th, and 10th hours of incubation (p < 0.05). VRE was the only bacteria that PRP and PPP showed limited activity against. When compared to PPP, PRP showed higher activity against MRSA, K. pneumoniae, and P. aeruginosa. However, the differences between PRP and PPP were statistically significant only against MRSA and P. aeruginosa at the first hour of incubation.
Conclusions:
Emerging PRP and other platelet-derived products seem to be promising alternative tools besides antibiotic treatment, debridement, negative pressure wound therapy, hyperbaric oxygen therapy, and other treatment options for treating diabetic foot infections.
Insights
Platelet-rich plasma (PRP) shows in vitro antibacterial activity against common wound pathogens like MRSA. This suggests PRP could be a promising localized treatment for infected wounds, including diabetic foot infections.
Area of Science:
- Wound Healing Research
- Antimicrobial Therapy
- Regenerative Medicine
Background:
- Diabetic foot infections are polymicrobial with high antimicrobial resistance.
- These infections lead to significant morbidity, mortality, and healthcare costs.
- Localized treatment options are needed to complement existing therapies.
Purpose of the Study:
- To evaluate the in vitro antibacterial activity of platelet-rich plasma (PRP).
- To assess PRP's efficacy against key multidrug-resistant bacteria found in wound infections.
- To support the development of PRP for clinical trials in infected wounds.
Main Methods:
- In vitro comparison of autologous PRP, platelet-poor plasma (PPP), and PBS.
- Testing against methicillin-resistant Staphylococcus aureus (MRSA), VRE, ESBL-producing K. pneumoniae, and CR-Pseudomonas aeruginosa.
- Assessment of bacterial growth inhibition and antimicrobial susceptibility.
Main Results:
- PRP and PPP significantly suppressed MRSA, K. pneumoniae, and P. aeruginosa growth.
- PRP demonstrated greater antibacterial activity than PPP against MRSA, K. pneumoniae, and P. aeruginosa.
- Limited activity was observed against vancomycin-resistant Enterococcus (VRE).
Conclusions:
- Platelet-rich plasma shows potential as an adjunct therapy for infected wounds.
- PRP may offer a novel localized treatment strategy for diabetic foot infections.
- Further clinical investigation is warranted for platelet-derived products in wound care.
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