Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Base Pairing02:27

DNA Base Pairing

33.7K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
33.7K
DNA Base Pairing02:27

DNA Base Pairing

33.0K
33.0K
Thermometers and Temperature Scales01:22

Thermometers and Temperature Scales

7.8K
Any physical property that depends consistently and reproducibly on temperature can be used as the basis of a thermometer. For example, volume increases with temperature for most substances. This property is the basis for the common alcohol thermometer and the original mercury thermometers. Other properties used to measure temperature include electrical resistance, color, and the emission of infrared radiation.
As many physical properties depend on temperature, the variety of thermometers is...
7.8K
Gas Thermometers and the Kelvin Scale01:22

Gas Thermometers and the Kelvin Scale

6.5K
The definition of temperature in terms of molecular motion suggests that there should be a lowest possible temperature, where the average kinetic energy of molecules is zero (or the minimum allowed by quantum mechanics). Experiments confirm the existence of such a temperature, called absolute zero. An absolute temperature scale is one whose zero point is absolute zero. Such scales are convenient in science because several physical quantities, such as the volume of an ideal gas, are directly...
6.5K
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

93.7K
93.7K
DNA Helicases00:55

DNA Helicases

24.2K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A nanowire-based fluorescent sensor for detecting hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) dyshomeostasis in cell body and synapse of Alzheimer's disease (AD) cell model.

Talanta·2026
Same author

Retina-Inspired Bi-Based Terahertz Photonic Neuromorphic Devices.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

A highly sensitive ratiometric fluorescence pH probe for extracellular pH in mouse brain slice.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

DiCleavePlus: A Transformer-Based Model to Detect Human Dicer Cleavage Sites Within Cleavage Patterns.

Genes to cells : devoted to molecular & cellular mechanisms·2025
Same author

Visualizing Endoplasmic Reticulum Stress and Autophagy in Alzheimer's Model Cells by a Peroxynitrite-Responsive AIEgen Fluorescent Probe.

ACS chemical neuroscience·2025
Same author

Simultaneous Imaging of pH and Peroxynitrite in the Endoplasmic Reticulum and Mitochondria: Revealing Organelle Interactions in Alzheimer's Disease Pathogenesis.

Analytical chemistry·2024

Related Experiment Video

Updated: Feb 11, 2026

A Method to Fabricate Disconnected Silver Nanostructures in 3D
05:45

A Method to Fabricate Disconnected Silver Nanostructures in 3D

Published on: November 27, 2012

14.2K

DNA nanostructure-based fluorescence thermometer with silver nanoclusters.

Congcong Bu1,2, Lixuan Mu1, Xingxing Cao1,2

  • 1Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.

Nanotechnology
|April 28, 2018
PubMed
Summary

Researchers developed DNA nanostructure fluorescence thermometers. These thermometers use silver nanoclusters and DNA to detect temperature changes, enabling precise measurements in biological systems like single cells.

More Related Videos

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

1.5K
Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

9.7K

Related Experiment Videos

Last Updated: Feb 11, 2026

A Method to Fabricate Disconnected Silver Nanostructures in 3D
05:45

A Method to Fabricate Disconnected Silver Nanostructures in 3D

Published on: November 27, 2012

14.2K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

1.5K
Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

9.7K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Accurate temperature monitoring is crucial for understanding cellular processes.
  • Existing thermometers face limitations in nanoscale biological environments.
  • DNA nanostructures offer a versatile platform for biosensing applications.

Purpose of the Study:

  • To design and fabricate novel DNA nanostructure-based fluorescence thermometers.
  • To investigate the temperature-sensing mechanism based on silver nanocluster-DNA interactions.
  • To evaluate the performance and potential applications of these thermometers in biological systems.

Main Methods:

  • Synthesis of fluorescent silver nanoclusters (AgNCs).
  • Assembly of DNA nanostructures incorporating G-rich DNA and a temperature-sensitive DNA stem-loop.
  • Characterization of fluorescence emission modulation in response to temperature variations.
  • Tuning the thermometer's response range by altering the DNA stem-loop's melting temperature.

Main Results:

  • Demonstrated sensitive fluorescence modulation of AgNCs at 636 nm due to temperature-dependent distance changes between AgNCs and G-rich DNA.
  • Achieved a high temperature sensitivity of -3.6%/°C for red emission, outperforming green emission.
  • Successfully adjusted the operational temperature range by modifying the DNA stem-loop's properties.

Conclusions:

  • Developed novel, highly sensitive DNA nanostructure-based fluorescence thermometers.
  • These thermometers offer a promising tool for nanoscale temperature measurements in biological systems, including single cells.
  • The tunable nature of the DNA nanostructure allows for customization of the sensing range.