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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Versatile Platform for Nanoparticle Surface Bioengineering Based on SiO2-Binding Peptide and Proteinaceous

Victoria O Shipunova1,2,3, Ivan V Zelepukin1,2,3, Oleg A Stremovskiy1

  • 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry , Russian Academy of Sciences , 16/10 Miklukho-Maklaya Street , Moscow 117997 , Russia.

ACS Applied Materials & Interfaces
|April 28, 2018
PubMed
Summary

A novel nanoparticle biomodification method uses a peptide and protein adaptor system for rapid, controlled surface coupling of biomolecules. This approach preserves biomolecule function and enhances targeted cancer cell delivery for theranostics.

Keywords:
BarnaseBarstarDARPinHER2/neubioengineeringcancer cellsnanoparticle modificationtargeted delivery

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Area of Science:

  • Nanotechnology
  • Biotechnology
  • Materials Science

Background:

  • Current nanoparticle surface coupling methods have limitations including low yield, poor biomolecule orientation, and steric hindrance.
  • These limitations hinder the application of nanostructures in theranostics and personalized medicine.

Purpose of the Study:

  • To develop a rapid, versatile nanoparticle biomodification method that overcomes the limitations of existing techniques.
  • To enable controlled surface coupling of biomolecules, preserving their orientation and function for enhanced nanomedical applications.

Main Methods:

  • Developed a biomodification strategy using a SiO2-binding peptide and the Barnase*Barstar protein pair as a molecular glue.
  • Demonstrated the method by coupling nanoparticles with DARPin9.29 and 4D5scFv molecules targeting HER2/neu oncomarker.
  • Evaluated nanoparticle targeting of HER2/neu-overexpressing cancer cells and interaction in biological fluids like whole blood.

Main Results:

  • The novel method achieved rapid biomodification (minutes), preserving biomolecule orientation and function.
  • Successfully demonstrated selective immunotargeting of modified nanoparticles to HER2/neu-overexpressing cancer cells.
  • The method preserved the biological activity of attached biomolecules, unlike conventional carbodiimide conjugation methods.

Conclusions:

  • The developed nanoparticle biomodification platform offers a versatile and efficient alternative to traditional chemical conjugation.
  • This method shows significant promise for advancing nanobiotechnology, theranostics, and clinical applications by enabling precise control over nanoparticle surface functionalization.
  • The platform facilitates the development of nanoparticles capable of selective antigen interaction in complex biological environments.