Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.7K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.7K
Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.6K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.6K
Embryonic Stem Cells00:57

Embryonic Stem Cells

5.2K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
5.2K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.1K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.1K
Dimensional Analysis03:40

Dimensional Analysis

65.2K
Dimensional analysis, also known as the factor label method, is a versatile approach for mathematical operations. The main principle behind this approach is: the units of quantities must be subjected to the same mathematical operations as their associated numbers. This method can be applied to computations ranging from simple unit conversions to more complex and multi-step calculations involving several different quantities and their units.
Conversion Factors and Dimensional Analysis
The unit...
65.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tmem2 Deficiency Leads to Enamel Hypoplasia and Soft Enamel in Mouse.

Journal of dental research·2023
Same author

Role of Heparan Sulfate in Vasculogenesis of Dental Pulp Stem Cells.

Journal of dental research·2022
Same author

Fabrication of Vascularized DPSC Constructs for Efficient Pulp Regeneration.

Journal of dental research·2021
Same author

VE-Cadherin and Anastomosis of Blood Vessels Formed by Dental Stem Cells.

Journal of dental research·2020
Same author

Gold Nanoparticles Inhibit Matrix Metalloproteases without Cytotoxicity.

Journal of dental research·2015
Same author

Expression of CD44 splicing isoforms in lung cancers: dominant expression of CD44v8-10 in non-small cell lung carcinomas.

International journal of oncology·1998

Related Experiment Video

Updated: Feb 11, 2026

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
03:45

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

3.3K

Pulp Regeneration by 3-dimensional Dental Pulp Stem Cell Constructs.

Y Itoh1,2, J I Sasaki1, M Hashimoto3

  • 11 Department of Biomaterials Science, Osaka University Graduate School of Dentistry, Osaka, Japan.

Journal of Dental Research
|April 28, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed scaffold-free 3D dental pulp stem cell (DPSC) constructs for regenerating pulpless teeth. These constructs successfully formed vascularized pulp-like tissue in vivo, showing potential for novel dental regeneration therapies.

Keywords:
biomaterialscell differentiationendodonticsodontoblastsregenerative medicinetissue engineering

More Related Videos

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
07:32

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

Published on: September 25, 2021

3.6K
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

27.3K

Related Experiment Videos

Last Updated: Feb 11, 2026

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells
03:45

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

3.3K
In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
07:32

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

Published on: September 25, 2021

3.6K
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

27.3K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Dental Research

Background:

  • Dental pulp regeneration aims to restore vitality to pulpless teeth.
  • Current tissue engineering approaches face challenges like long regeneration times and scaffold-related risks (inflammation, infection).
  • Developing scaffold-free methods is crucial for safer and more efficient dental pulp regeneration.

Purpose of the Study:

  • To fabricate and evaluate scaffold-free 3-dimensional (3D) cell constructs using dental pulp stem cells (DPSCs) for pulp regeneration.
  • To assess the viability and self-organization capabilities of these 3D DPSC constructs in vitro.
  • To investigate the in vivo efficacy of 3D DPSC constructs for regenerating pulp-like tissue within a root canal environment.

Main Methods:

  • Fabrication of 3D DPSC constructs using DPSCs and a thermoresponsive hydrogel.
  • In vitro assessment of DPSC viability and self-organization within the constructs.
  • In vivo implantation of 3D DPSC constructs into human root canals, which were then subcutaneously implanted into immunodeficient mice.
  • Histological analysis to evaluate tissue formation, vascularization, and cell differentiation.

Main Results:

  • 3D DPSC constructs maintained DPSC viability and demonstrated self-organization potential in vitro.
  • In vivo implantation resulted in the formation of blood vessel-rich, pulp-like tissues within the root canal after 6 weeks.
  • Transplanted DPSCs differentiated into odontoblast-like cells upon contact with dentin, and vascularization was observed.
  • Successful regeneration occurred without the need for scaffolds or exogenous growth factors.

Conclusions:

  • Scaffold-free 3D DPSC constructs possess the self-organizing ability required for successful pulp regeneration in vivo.
  • This novel technology enables the creation of vascularized pulp-like tissue using DPSCs, offering a promising alternative to current regenerative therapies.
  • The ability to create customizable DPSC constructs paves the way for advanced treatments for pulpless teeth.