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Gold Nanoparticle Synthesis
Published on: July 10, 2021
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Spectrofluorimetric method for atenolol determination based on gold nanoparticles
Esam Bakir1,2, Mohamed Gouda1,3, Ahmed Alnajjar1
1Department of Chemistry, College of Science, King Faisal University, Hofuf, Saudi Arabia.
Acta Pharmaceutica (Zagreb, Croatia)
|April 28, 2018
Summary
A new spectrofluorimetric method using gold nanoparticles (AuNPs) offers a simple and sensitive way to determine atenolol (ATE). This technique accurately quantifies atenolol in pharmaceutical formulations.
Area of Science:
- Analytical Chemistry
- Nanotechnology
Background:
- Atenolol is a widely used beta-blocker for cardiovascular conditions.
- Accurate quantification of atenolol in pharmaceutical formulations is crucial for quality control.
Purpose of the Study:
- To develop a simple, sensitive, and reliable spectrofluorimetric method for atenolol determination.
- To utilize gold nanoparticles (AuNPs) as a fluorescent probe for atenolol detection.
Main Methods:
- Spectrofluorimetric analysis leveraging the quenching effect of atenolol on gold nanoparticle photoluminescence.
- Optimization of reaction parameters including solvent, pH, and surfactant concentration.
- Preliminary validation according to International Council for Harmonisation (ICH) guidelines.
Main Results:
- A linear correlation was observed for atenolol concentrations between 1.0-10 mg mL⁻¹ (R² = 0.999).
- The limit of detection (LOD) and limit of quantitation (LOQ) were determined to be 0.87 and 2.64 mg mL⁻¹, respectively.
- High recovery rates (98.7-100.0%) were achieved in spiked pharmaceutical samples.
Conclusions:
- The developed gold nanoparticle-based spectrofluorimetric method is effective for the quantitative determination of atenolol.
- The method demonstrates high sensitivity, accuracy, and applicability to real pharmaceutical samples.
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