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Thermal strain is a concept that arises when we consider how temperature changes affect structures. Unlike the conventional assumption that structures remain constant under load, real-world scenarios often involve temperature fluctuations that can significantly impact these structures. Consider a homogeneous rod with a uniform cross-section resting freely on a flat horizontal surface. If the rod's temperature increases, the rod elongates. This elongation is proportional to the temperature...
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Strain energy is a fundamental concept in the field of materials science and structural engineering, describing the energy absorbed by a material or structure when it is deformed under load.
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Rhodococcus strains as source for ene-reductase activity.

Bi-Shuang Chen1,2, Rosario Médici1, Michelle P van der Helm1

  • 1Biocatalysis, Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands.

Applied Microbiology and Biotechnology
|April 30, 2018
PubMed
Summary
This summary is machine-generated.

Rhodococcus strains exhibit (S)-selectivity in asymmetric reduction of C=C bonds, differing from most organisms. This finding highlights their potential for producing valuable chiral compounds.

Keywords:
Asymmetric reductionEnantioselectivityEne-reductaseRhodococcus

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Area of Science:

  • Biotechnology
  • Enzyme catalysis
  • Organic chemistry

Background:

  • Rhodococcus strains are versatile microorganisms capable of metabolizing diverse compounds.
  • Asymmetric reduction of carbon-carbon double bonds (C=C) is crucial for synthesizing chiral building blocks.
  • Exploring microbial capabilities for enantioselective synthesis is an active area of research.

Purpose of the Study:

  • To investigate the ene-reductase activity in Rhodococcus strains for asymmetric reduction.
  • To evaluate the potential of Rhodococcus species in producing chiral compounds.
  • To characterize novel ene-reductases from Rhodococcus rhodochrous.

Main Methods:

  • Screening of Rhodococcus rhodochrous and R. erythropolis strains for ene-reductase activity.
  • Substrate screening using whole cells with activated ketones, aldehydes, imides, and nitro-compounds.
  • Heterologous expression of putative ene-reductases in Escherichia coli and subsequent protein purification.

Main Results:

  • All tested Rhodococcus strains demonstrated (S)-selectivity towards ketoisophorone reduction.
  • This observed (S)-selectivity contrasts with the typical (R)-selectivity seen in other microorganisms.
  • Purified ene-reductase from R. rhodochrous ATCC 17895 exhibited characteristics of class 3 ene-reductases.

Conclusions:

  • Rhodococcus strains possess valuable ene-reductase activity for enantioselective synthesis.
  • These microorganisms offer a promising biocatalytic platform for the production of (S)-chiral compounds.
  • The identified ene-reductases are potential tools for industrial applications in chiral chemistry.