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Dispersion of Nanomaterials in Aqueous Media: Towards Protocol Optimization
Published on: December 25, 2017
Engineered alcohol oxidases catalyse transesterification in aqueous media without competing hydrolysis
Bin Wu1, Yunjian Ma2,3, Chenhao Feng1
1School of Food Science and Engineering, South China University of Technology, Guangzhou, China.
None:
Transesterification reactions are fundamental transformations in organic chemistry, yet performing them in aqueous media is challenging because of the competing hydrolysis reaction. In this study, we describe a mutant of alcohol oxidase from Phanerochaete chrysosporium (PcAOx-VPN) that also exhibits transesterification activity. Moreover, PcAOx-VPN displays no detectable hydrolytic activity, owing to its hydrophobic active site, which effectively excludes water. These characteristics make PcAOx-VPN a promising catalyst for transesterification reactions in aqueous media, a context that is typically compromised by competing hydrolysis.
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