Immunolocalization and changes of 17beta-estradiol during ovarian development of Chinese mitten crab Eriocheir...

17beta-estradiol (E₂) is important for crustacean ovarian development. This study aims to investigate the distribution and change pattern of E₂ in the ovary, hepatopancreas, thoracic ganglion and brain ganglion as well as Vg-mRNA expression level during ovarian development of Chinese mitten crab Eriocheir sinensis. Results showed that strongly positive signals of E₂ were mainly distributed in follicle cells of ovaries for all developmental stages as well as oocyte cytoplasm of stages III to V ovaries. In hepatopancreas, the E₂-positive signal was mainly detected in the cytoplasm and nucleus of fibrillar cells and the nucleus of resorptive cells, while the maximum fluorescence intensity was observed in stage III hepatopancreas. On the contrary, the E₂ immunoreactivities in nervous tissues were relatively stable during ovarian development. Moreover, the changing pattern of E₂ concentration was similar within hemolymph, ovary and hepatopancreas during the ovarian development. From stages I to III, the E₂ content in three tissues increased significantly, then decreased gradually until stage V. As for the Vg-mRNA expression level in hepatopancreas and ovaries, an increasing trend was found in ovaries but no significant difference was detected during the period of ovarian stages III to V. Hepatopancreatic Vg-mRNA expression level increased significantly during stages I to IV and dramatically decreased at stage V. In conclusion, our study suggests that ovary, hepatopancreas, hemolymph and nervous tissues are the target organs of E₂ in E. sinensis and E₂ concentrations in different tissues are closely related to vitellogenesis in ovary and hepatopancreas during ovarian development.