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Related Experiment Videos

Streptococcus mutans gtfA gene specifies sucrose phosphorylase.

R R Russell1, H Mukasa, A Shimamura

  • 1Royal College of Surgeons of England, Downe, Kent, United Kingdom.

Infection and Immunity
|October 1, 1988
PubMed
Summary

The Streptococcus mutans GTF-A enzyme, when expressed in E. coli, produces alpha-D-glucose-1-phosphate from sucrose. This enzyme is identified as sucrose phosphorylase, crucial for carbohydrate metabolism.

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Area of Science:

  • Biochemistry
  • Enzymology
  • Microbiology

Background:

  • Streptococcus mutans utilizes sucrose for glucan synthesis, contributing to dental caries.
  • The gtfA gene encodes a key enzyme involved in sucrose metabolism.

Purpose of the Study:

  • To characterize the product of the gtfA gene enzyme from Streptococcus mutans.
  • To identify the enzymatic activity of the GTF-A enzyme.

Main Methods:

  • Purification of the enzyme encoded by the gtfA gene from recombinant Escherichia coli.
  • Analysis of the ethanol-insoluble product using 13C and 31P nuclear magnetic resonance (NMR) spectroscopy.

Main Results:

  • The enzyme produced an ethanol-insoluble product from sucrose.

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  • Nuclear magnetic resonance analysis identified the product as alpha-D-glucose-1-phosphate.

    • Conclusions:

    • The GTF-A enzyme from Streptococcus mutans functions as sucrose phosphorylase (sucrose:orthophosphate alpha-D-glucosyltransferase [EC 2.4.1.7]).
    • This finding clarifies the enzymatic mechanism of sucrose metabolism in S. mutans.