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A 2',4'-Bridged Nucleic Acid Containing 2-Pyridone as a Nucleobase: Efficient Recognition of a C⋅G Interruption by
Satoshi Obika1, Yoshiyuki Hari1, Mitsuaki Sekiguchi1
1Graduate School of Pharmaceutical Sciences Osaka University 1-6 Yamadaoka, Suita, Osaka 565-0871 (Japan) Fax: (+81) 6-6879-8204.
Angewandte Chemie (International Ed. in English)
|May 2, 2018
Abstract:
Significantly enhanced binding affinity to C⋅G base pairs without loss of sequence selectivity is achieved by using a nucleotide containing a 2-pyridone and a 2'-O,4'-C-methylene-bridged nucleic acid analogue (PB , see picture). The degree of stabilization of the triplex formed enables C⋅G interruptions in a homopurine⋅homopyrimidine double-stranded DNA to be detected.