Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Replication02:40

DNA Replication

60.0K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
60.0K
The DNA Replication Fork01:02

The DNA Replication Fork

41.2K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
41.2K
The DNA Replication Fork01:02

The DNA Replication Fork

18.6K
18.6K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

5.7K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
5.7K
Chromosome Replication02:31

Chromosome Replication

10.8K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.8K
Replication in Prokaryotes02:35

Replication in Prokaryotes

99.1K
Overview
99.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimising DNA origami assembly by reducing off-target interactions.

Nature communications·2026
Same author

Selection and Characterization of SARS-CoV-2 Spike Binding Clickmers.

Chembiochem : a European journal of chemical biology·2026
Same author

High-Throughput Determination of Infectious Virus Titers by Kinetic Measurement of Infection-Induced Changes in Cell Morphology.

International journal of molecular sciences·2024
Same author

A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein.

International journal of molecular sciences·2024
Same author

A rhythmically pulsing leaf-spring DNA-origami nanoengine that drives a passive follower.

Nature nanotechnology·2023
Same author

Reconfigurable Nanopolygons Made of DNA Catenanes.

Bioconjugate chemistry·2023

Related Experiment Video

Updated: Feb 11, 2026

Molecular Entanglement and Electrospinnability of Biopolymers
07:59

Molecular Entanglement and Electrospinnability of Biopolymers

Published on: September 3, 2014

15.1K

Functionalized DNA: A New Replicable Biopolymer.

Oliver Thum1, Stefan Jäger1, Michael Famulok1

  • 1Kekulé-Institut für Organische Chemie und Biochemie Universität Bonn Gerhard-Domagk-Strasse 1, 53121 Bonn (Germany) Fax: (+49) 228-735388.

Angewandte Chemie (International Ed. in English)
|May 2, 2018
PubMed
Summary
This summary is machine-generated.

Researchers created functionalized DNA (fDNA) by adding amino acid-like residues to each base. This novel biopolymer can be amplified using polymerase chain reaction for in vitro selection techniques.

Keywords:
combinatorial chemistryin vitro selectionnucleotidesoligonucleotides

More Related Videos

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

23.1K
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

19.3K

Related Experiment Videos

Last Updated: Feb 11, 2026

Molecular Entanglement and Electrospinnability of Biopolymers
07:59

Molecular Entanglement and Electrospinnability of Biopolymers

Published on: September 3, 2014

15.1K
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

23.1K
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

19.3K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Traditional DNA and peptides have distinct functional group repertoires.
  • In vitro selection techniques require versatile molecular building blocks.

Purpose of the Study:

  • To create a novel functionalized DNA (fDNA) by incorporating amino acid-like residues onto the DNA backbone.
  • To explore the potential of fDNA as a new class of biopolymers for advanced molecular applications.

Main Methods:

  • Enzymatic polymerization of base-modified nucleoside triphosphates.
  • Generation of functionalized DNA (fDNA) with amino acid-like residues on each base.
  • Utilizing fDNA as templates for polymerase chain reaction (PCR) amplification.

Main Results:

  • Successfully synthesized fDNA where each base mimics peptide functional groups.
  • Demonstrated that fDNA can serve as a template for PCR amplification.
  • Established fDNA as a novel biopolymer for in vitro selection.

Conclusions:

  • Functionalized DNA represents a significant advancement in biopolymer design.
  • fDNA offers a unique platform for expanding the capabilities of in vitro selection and other molecular techniques.