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Related Experiment Video

Updated: Feb 11, 2026

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

High spatial resolution nanoslit SERS for single-molecule nucleobase sensing.

Chang Chen^1,2, Yi Li^3,4, Sarp Kerman^3,5

¹imec, Kapeldreef 75, 3001, Leuven, Belgium. chang.chen@imec.be.

Nature Communications

|May 2, 2018

Summary

This summary is machine-generated.

This study introduces a novel plasmonic nanoslit nanopore for single-molecule DNA analysis. It enables sensitive, high-resolution identification of DNA nucleobases using surface-enhanced Raman spectroscopy (SERS).

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Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

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Area of Science:

Nanotechnology and Spectroscopy
Single-molecule analysis
Biophysics

Background:

Solid-state nanopores offer scalable DNA analysis platforms.
Current ionic sensing methods face limitations in sensitivity and spatial resolution for real-time nucleobase identification.
Optical spectroscopy presents a promising alternative sensing strategy.

Purpose of the Study:

To investigate the potential of an optically engineered nanopore (plasmonic nanoslit) for single-molecule DNA analysis.
To combine surface-enhanced Raman spectroscopy (SERS) with nanopore fluidics for direct nucleobase identification.
To assess the sensitivity and spatial resolution of this combined approach.

Main Methods:

Utilized an optically engineered elongated nanopore structure: a plasmonic nanoslit.
Employed single-molecule surface-enhanced Raman spectroscopy (SERS) for molecular fingerprinting.
Combined SERS with nanopore fluidics for electrokinetic capture and identification of DNA analytes.

Main Results:

Achieved direct Raman spectroscopic fingerprinting of four DNA nucleobases.
Observed asynchronous spectroscopic behavior of individual and incorporated nucleobases within DNA strands during stochastic fluctuations.
Demonstrated single-molecule sensitivity and sub-nanometer spatial resolution.

Conclusions:

Plasmonic nanoslit SERS coupled with nanopore fluidics provides a viable strategy for sensitive, high-resolution DNA nucleobase identification.
The method overcomes limitations of established ionic sensing techniques.
This approach shows significant promise for advancing single-molecule DNA analysis.