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Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico

Karla Saavedra-Rodriguez1, Farah Vera Maloof1, Corey L Campbell1

  • 1Arthropod-borne and Infectious Disease Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.

Scientific Reports
|May 2, 2018
PubMed

Insights

Pyrethroid insecticide resistance in Aedes aegypti mosquitoes is increasing due to genetic mutations. A new mutation, V410L, has emerged in Mexico, alongside existing resistance genes, complicating control efforts.

Area of Science:

  • Medical Entomology
  • Molecular Entomology
  • Insecticide Resistance

Background:

  • Aedes aegypti mosquitoes transmit dengue, Zika, and chikungunya viruses, with limited treatment options.
  • Insecticidal control is challenged by widespread pyrethroid resistance in mosquito populations.
  • Knockdown resistance (kdr) mutations in the voltage-gated sodium channel gene (vgsc) are a primary driver of pyrethroid resistance.

Purpose of the Study:

  • To investigate the prevalence and evolution of the V410L vgsc mutation in Mexican Aedes aegypti populations.
  • To understand the interaction of V410L with other known kdr mutations (F1,534C, V1,016I) in conferring insecticide resistance.
  • To inform resistance management strategies for Aedes aegypti control.

Main Methods:

  • Screening of 25 historical Aedes aegypti collections from Mexico for the V410L mutation.
  • Analysis of mutation frequencies over the last 16 years.
  • Comparison of V410L prevalence with co-occurring mutations F1,534C and V1,016I.

Main Results:

  • The V410L mutation was detected in Mexican Aedes aegypti populations, with the first heterozygote identified in 2002.
  • V410L frequencies have increased over the past 16 years.
  • The emergence and rise of V410L occurred alongside increasing frequencies of V1,016I and F1,534C mutations.

Conclusions:

  • The V410L mutation is spreading in Mexican Aedes aegypti populations and co-evolving with other kdr mutations.
  • Understanding these specific vgsc mutations and their interactions is crucial for effective insecticide resistance management.
  • This knowledge will aid in predicting and developing sustainable strategies to control arbovirus vectors.

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