Related Experiment Video

Updated: Feb 11, 2026

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

25.1K

A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7

Fang-Zhou Qiu1,2, Xin-Xin Shen2, Meng-Chuan Zhao3

  • 1Hebei Medical University, Shijiazhuang, 050031, Hebei, China.

Virology Journal
|May 3, 2018
PubMed
Abstract

Keywords:
ClinicalHAdVPneumoniaTriplex quantitative real-time PCR

More Related Videos

Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay09:21

Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay

Published on: June 17, 2010

50.6K
Analysis of Gene Expression in Emerald Ash Borer Agrilus planipennis Using Quantitative Real Time-PCR11:22

Analysis of Gene Expression in Emerald Ash Borer Agrilus planipennis Using Quantitative Real Time-PCR

Published on: May 4, 2010

11.1K

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
65.3K
ortho–para-Directing Activators: –CH3, –OH, –⁠NH2, –OCH301:11

ortho–para-Directing Activators: –CH3, –OH, –⁠NH2, –OCH3

All ortho–para directors, excluding halogens, are activating groups. These groups donate electrons to the ring, making the ring carbons electron-rich. Consequently, the reactivity of the aromatic ring towards electrophilic substitution increases. For instance, the nitration of anisole is about 10,000 times faster than the nitration of benzene. The electron-donating effect of the methoxy group in anisole activates the ortho and para positions on the ring and stabilizes the corresponding...
7.5K
PCR01:32

PCR

Overview
238.5K
Real Number System01:27

Real Number System

The real number system includes all numbers used for counting, measuring, and comparing quantities. Natural numbers are the basic counting numbers: 1, 2, 3, and so on. Integers expand this set by including zero and negative whole numbers: ..., –3, –2, –1, 0, 1, 2, .... Rational numbers are those that can be expressed as the ratio of two integers m/n, where n≠0. This includes fractions like 1/2​, integers such as 46, and decimals that terminate, such as 0.17, or...
1.2K
meta-Directing Deactivators: –NO2, –CN, –CHO, –⁠CO2R, –COR, –CO2H01:13

meta-Directing Deactivators: –NO2, –CN, –CHO, –⁠CO2R, –COR, –CO2H

All meta-directing substituents are deactivating groups. These substituents withdraw electrons from the aromatic ring, making the ring less reactive toward electrophilic substitution. For example, the nitration of nitrobenzene is 100,000 times slower than that of benzene because of the deactivating effect of the nitro group. The first step in an electrophilic aromatic substitution is the addition of an electrophile to form a resonance-stabilized carbocation. The energy diagrams for...
6.7K
Real Number Operations01:27

Real Number Operations

The concept of real numbers includes all the values that can be represented on a continuous number line. The system began with basic counting values used for enumeration. It later expanded to include values that represent the absence of quantity and opposites of the counting values. When situations required expressing parts of a whole or dividing quantities evenly, values capable of representing such proportions were developed. When written using decimal notation, these values can end or repeat...
339