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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
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Cloned and expressed human Fc receptor for IgG mediates anti-CD3-dependent lymphoproliferation

G A Peltz1, M L Trounstine, K W Moore

  • 1Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304.

Journal of Immunology (Baltimore, Md. : 1950)
|September 15, 1988
PubMed

We have utilized gene transfer experiments to investigate the role of a human monocyte receptor for IgG (Fc gamma RII) in mouse IgG1 anti-CD3 (Leu 4)-induced lymphoproliferation in vitro. Mouse Ltk- cells expressing human Fc gamma RII or a mutant of Fc gamma RII lacking the entire cytoplasmic domain of the receptor mediate anti-CD3-induced lymphoproliferation in cultures of adherent cell-depleted human PBMC. Expression of an Fc gamma RII mutant lacking transmembrane and cytoplasmic domains (soluble Fc gamma RII) in COS7 cells yielded a secreted receptor which retained affinity for IgG, even in the absence of the mutant receptor's N-linked oligosaccharides. Soluble Fc gamma RII inhibits rosette formation by human IgG-sensitized RBC and the Fc gamma RII-bearing cell line K562, but does not sitmulate anti-CD3-induced lymphoproliferation under the conditions tested.

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