Related Experiment Video
Updated: Feb 11, 2026

Quantification of Circular RNAs Using Digital Droplet PCR
Published on: September 16, 2022
Droplet Digital™ PCR Next-Generation Sequencing Library QC Assay
1Digital Biology Center, Bio-Rad Laboratories, Pleasanton, CA, USA. nick_heredia@bio-rad.com.
Abstract:
Digital PCR is a valuable tool to quantify next-generation sequencing (NGS) libraries precisely and accurately. Accurately quantifying NGS libraries enable accurate loading of the libraries on to the sequencer and thus improve sequencing performance by reducing under and overloading error. Accurate quantification also benefits users by enabling uniform loading of indexed/barcoded libraries which in turn greatly improves sequencing uniformity of the indexed/barcoded samples. The advantages gained by employing the Droplet Digital PCR (ddPCR™) library QC assay includes the precise and accurate quantification in addition to size quality assessment, enabling users to QC their sequencing libraries with confidence.
Related Concept Videos
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
PCR
Cis-regulatory Sequences
Sequences
Sanger Sequencing
Arithmetic Sequences

