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Oli2go: an automated multiplex oligonucleotide design tool.

Michaela Hendling1, Stephan Pabinger1, Konrad Peters2

  • 1AIT Austrian Institute of Technology, Center for Health, and Bioresources, Molecular Diagnostics, Muthgasse 11, 1190 Vienna, Austria.

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|May 3, 2018
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Summary

Oli2go is an automated multiplex oligonucleotide design tool. It combines multiple design steps, including specificity and dimer checks, into one efficient solution for high-quality results.

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Area of Science:

  • Molecular Biology
  • Bioinformatics

Background:

  • Accurate oligonucleotide design is crucial for molecular biology techniques like PCR and microarrays.
  • Existing web tools often perform only partial oligonucleotide design workflows.

Purpose of the Study:

  • To introduce Oli2go, a user-friendly, automated multiplex oligonucleotide design tool.
  • To provide an all-in-one solution for primer and probe design, including specificity and dimer checks.

Main Methods:

  • Oli2go integrates multiple design steps into a single automated process.
  • Specificity checks are performed against a broad range of organisms and sequence databases.
  • All-against-all dimer checks are conducted for primers and probes.

Main Results:

  • Oli2go offers an efficient, user-friendly, and fully automated multiplex oligonucleotide design.
  • The tool ensures high specificity by checking against diverse biological and environmental sequences.
  • Comprehensive dimer checks enhance the reliability of designed oligonucleotides.

Conclusions:

  • Oli2go streamlines the oligonucleotide design process, improving efficiency and quality.
  • The tool's broad specificity checks are vital for complex multiplex applications.
  • Oli2go is freely accessible, supporting researchers in molecular biology and bioinformatics.