Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem Cells
Yinxiang Wang1,2, Jessica Aijia Liu2, Keith K H Leung1
1Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
Stem Cells International
|May 4, 2018
Summary
Induced pluripotent stem (iPS) cells were successfully generated from intramembranous osteoblasts. This study demonstrates the potential of reprogramming bone cells for regenerative medicine applications.
Area of Science:
- Stem Cell Biology
- Bone Biology
- Developmental Biology
Background:
- Previous research established reprogramming of endochondral bone cells into induced pluripotent stem (iPS) cells.
- The potential for reprogramming intramembranous bone cells into iPS cells remained unexplored.
Purpose of the Study:
- To investigate whether intramembranous bone cells can be reprogrammed into iPS cells.
- To characterize the pluripotency of iPS cells derived from intramembranous osteoblasts.
Main Methods:
- Utilized fluorescence-activated cell sorting to isolate homogenous intramembranous calvarial osteoblasts from transgenic mice.
- Employed retroviral transduction of Yamanaka factors (Oct4, Sox2, Klf4, c-Myc) to induce reprogramming.
- Analyzed gene expression profiles and assessed pluripotency through teratoma formation and chimera contribution.
Main Results:
- Osteoblasts showed early silencing of Osx1-GFP::Cre and late activation of Oct4-EGFP during reprogramming.
- Generated iPS cells exhibited gene expression patterns similar to embryonic stem cells.
- Demonstrated pluripotency via teratoma formation (all germ layers) and successful contribution to chimera embryos.
Conclusions:
- Successfully generated induced pluripotent stem (iPS) cells from intramembranous osteoblasts.
- This research expands the cell types amenable to reprogramming for potential therapeutic applications in bone regeneration.
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