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Updated: Feb 11, 2026

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
Effect of tissue staining in quantitative phase imaging
Sungbea Ban1, Eunjung Min2, Yujin Ahn1
1Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, Republic of Korea.
Abstract:
Quantitative phase imaging (QPI) is an emerging modality, which enables the identification of abnormalities in tissue based on optical properties. QPI can be applied to any biological specimen due to its label-free imaging capability, but its use in stained tissue is unclear. Here, we study the variability of QPI with the staining dye. Several tissues such as brain, heart and lung were stained with hematoxylin and eosin, and their optical properties compared at 550 and 730 nm. Our results showed that phase and scattering coefficients varied when QPI was used at the absorption wavelength of the staining dye. We also found that the variation of optical properties was dependent on tissue morphology.
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