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Updated: Feb 11, 2026

Quantitative 31P NMR Analysis of Lignins and Tannins
Published on: August 2, 2021
[¹H-NMR quantitative analysis and fingerprints of ginkgo diterpene lactone raw material]
Xia-Lin Chen1,2, Ting Geng1,2, Wen-Zhe Huang1,2
1Jiangsu Kanion Parmaceutical Co., Ltd., Lianyungang 222001, China.
Abstract:
Ginkgo diterpene lactone raw material, as a raw material for ginkgo diterpene lactone meglumine injection, is extracted and purified from ginkgo leaf. ¹H-NMR content determination method and fingerprint analysis method were respectively established for ginkgo diterpene lactone raw material. Content determination was conducted in 3 batches of samples by using ¹H-qNMR, and then the results were basically consistent with the results in HPLC method. Twenty-four proton peaks were identified as common fingerprint peaks, and the fingerprint peaks were identified by using the control product and NMR information. Furthermore, 10 batches of samples were analyzed by ¹H-NMR fingerprint. The similarities were all higher than 0.99 and the common peaks were identified with the reference standards. This method is easy, fast, with good precision, stability and repeatability and could provide basis and new ideas for quality evaluation of ginkgo diterpene lactone raw material and its preparations.
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