Related Experiment Video
Updated: Feb 11, 2026
11:13
Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
Published on: August 19, 2015
8.7K
Uniform polysaccharide composite microspheres with controllable network by microporous membrane emulsification
Hongwu Zhang1,2, Lan Zhao3, Yongdong Huang1
1State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.
Analytical and Bioanalytical Chemistry
|May 8, 2018
Summary
Researchers developed novel uniform polysaccharide composite microspheres for high-resolution chromatography. These tailor-made microspheres offer adjustable pore structures, enhancing separation resolution for biomolecules.
Area of Science:
- Chromatography
- Materials Science
- Biotechnology
Background:
- High resolution is crucial in preparative and analytical chromatography.
- Chromatographic media significantly influence separation efficiency.
- Tailor-made media with adjustable structures are increasingly important for specific applications.
Purpose of the Study:
- To develop novel uniform polysaccharide composite microspheres.
- To investigate the regulation of pore structure by adjusting polysaccharide composition and dextran molecular weight.
- To evaluate the separation resolution and mechanical strength of the composite microspheres.
Main Methods:
- Membrane emulsification technique using agarose and dextran solutions.
- Systematic adjustment of polysaccharide composition and dextran molecular weight.
- Characterization using Atomic Force Microscopy (AFM).
Main Results:
- Polysaccharide composite microspheres exhibited higher separation resolution than pure agarose microspheres.
- Pore size and distribution were controllable by varying agarose/dextran ratios and dextran molecular weight.
- Optimized compositions (e.g., 10% agarose/2% dextran T40) improved resolution for low molecular weight proteins.
- Enhanced mechanical strength and even pore distribution were observed.
Conclusions:
- Uniform polysaccharide composite microspheres offer tunable properties for high-resolution bioseparation.
- The developed microspheres demonstrate significant potential in chromatographic applications.
- This novel material advances the field of tailor-made chromatographic media.
Related Concept Videos
01:26Biosynthesis of Polysaccharides
713
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
713
03:35Classifying Matter by Composition
91.0K
Matter: Pure Substances and Mixtures
According to its composition, the matter can be classified into two broad categories — pure substances and mixtures.
A pure substance is a form of matter that has a constant composition throughout with uniform properties. For example, any sample of sucrose has the same composition and same physical properties, such as melting point, color, and sweetness, regardless of the source from which it is isolated.
A mixture is composed of two or...
According to its composition, the matter can be classified into two broad categories — pure substances and mixtures.
A pure substance is a form of matter that has a constant composition throughout with uniform properties. For example, any sample of sucrose has the same composition and same physical properties, such as melting point, color, and sweetness, regardless of the source from which it is isolated.
A mixture is composed of two or...
91.0K
01:19Uniform Distribution
6.1K
The uniform distribution is a continuous probability distribution of events with an equal probability of occurrence. This distribution is rectangular.
Two essential properties of this distribution are
Two essential properties of this distribution are
6.1K
02:26Protein Networks
4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
01:15Cellulose and Pectic Polysaccharides
5.0K
Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
As a cell matures, its cell wall specializes according to its type. For example, the...
5.0K
01:14Uniform Circular Motion
22.4K
Uniform circular motion is a specific type of motion in which an object travels in a circle with a constant speed. For example, any point on a propeller spinning at a constant rate is undergoing uniform circular motion. The second, minute, and hour hands of a watch also undergo uniform circular motion. It is hard to believe that points on these rotating objects are actually accelerating, even though the rotation rate is constant. To understand this, we must analyze the motion in terms of...
22.4K