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Updated: Feb 10, 2026

A Mouse Fetal Skin Model of Scarless Wound Repair
Published on: January 16, 2015
Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration
Ehsan Taghiabadi1,2, Bahareh Beiki2, Nasser Aghdami1
1Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Abstract:
Various natural and synthetic biomaterials have been applied as skin substitutes for regenerating damaged skin. Here, we describe a straightforward method for fabrication of a tissue-engineered skin substitute by seeding human fetal fibroblasts on acellular human amniotic membrane (HAM). Fetal fibroblasts are achieved from the skin of normal and non-macerated fetus of 11-14 weeks old after spontaneous pregnancy termination. Acellular HAM is obtained by separation of the outer membrane of the chorion and removing its epithelial cells.
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