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Updated: Feb 10, 2026

Mouse Round Spermatid Injection
Published on: January 26, 2024
[Application value of frozen-thawed mouse round spermatids in in vitro fertilization]
Hong Jiang1, Li Wang2, Cun-Li Wang1
1Center of Reproductive Medicine, The 105th Hospital of PLA, Hefei, Anhui 230031, China.
Objective:
To investigate the application value of the frozen-thawed round spermatids of the mouse in in vitro fertilization (IVF).
Methods:
Haploid spermatids of the mouse obtained in vitro were divided into a frozen-thawed and a fresh group and oocytes were collected from 6-8 weeks old female mice. After diamidino-phenyl-indole (DAPI) staining, the oocytes were subjected to intracytoplasmic round spermatid injection (ROSI), 259 in the frozen-thawed and 238 in the fresh group. Comparisons were made between the two groups in the capacities of fertilization and embryonic development.
Results:
The survival rate of the frozen-thawed haploid spermatids was (75.9 ± 2.3) %. No statistically significant differences were observed between the frozen-thawed and fresh groups after ROSI in the rates of fertilization (51.9 vs 55.7%, P >0.05), 2-cell embryos (51.0 vs 62.2%, P >0.05), 4-8-cell embryos (41.8 vs 42.9%, P >0.05), or morula-blastocysts (12.2 vs 21.4%, P >0.05).
Conclusions:
Frozen-thawed round spermatids of the mouse are similar to fresh ones in their capacities of fertilization and embryonic development.
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