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Employing metabolic engineered lipolytic microbial platform for 1-alkene one-step conversion
Juli Wang1, Haiying Yu2, Kun Zhu2
1CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Bioresource Technology
|May 9, 2018
Summary
This study engineered a microbial platform for producing 1-alkenes from hydrophobic sources, achieving the highest reported titers to date. This advancement holds promise for sustainable biofuel and chemical production.
Area of Science:
- Biotechnology
- Synthetic Biology
- Microbial Engineering
Background:
- 1-Alkenes are crucial chemical feedstocks and potential biofuels.
- Current production methods often rely on petrochemical sources.
- Developing sustainable biosynthetic routes is essential.
Purpose of the Study:
- To engineer a lipolytic microbial platform for 1-alkene production.
- To utilize hydrophobic substrates, such as fatty acids and oils, as the sole carbon source.
- To optimize 1-alkene yield and titer.
Main Methods:
- Cloning and expression of fatty acid decarboxylases (UndA and UndB) in Pseudomonas.
- Optimization of culturing conditions (temperature, pathway interruption).
- Genetic modifications including chromosomal integration, thioesterase expression, and pathway adjustments.
Main Results:
- Successful production of 1-alkenes using lauric acid and palm oil media.
- Initial titers of 177.8 mg/L (lauric acid) and 128.9 mg/L (palm oil) achieved with UndB expression.
- Enhanced titers reached 1102.6 mg/L (lauric acid) and 778.4 mg/L (palm oil) after further engineering.
Conclusions:
- Demonstrated the first lipolytic microbial platform for 1-alkene production.
- Achieved the highest reported 1-alkene titers to date.
- Validated the potential of engineered microbes for sustainable chemical and biofuel synthesis.
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