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Updated: Feb 10, 2026

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Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus

Fei Chen1, Liyuan Zhu1, Yiling Zhang1

  • 1School of Biology & Basic Medical Science, Soochow University, Suzhou, 215123, China.

Scientific Reports
|May 10, 2018
PubMed

Insights

Bombyx mori cypovirus (BmCPV) enters silkworm cells via endocytosis. Clathrin-mediated endocytosis is the primary entry route, suggesting it as a therapeutic target for silkworm cytoplasmic polyhedrosis.

Area of Science:

  • Virology
  • Cell Biology
  • Insect Pathology

Background:

  • Bombyx mori cypovirus (BmCPV) causes significant economic losses in the sericulture industry.
  • The cellular entry mechanism of BmCPV remains largely uncharacterized.

Purpose of the Study:

  • To elucidate the cellular entry pathway of BmCPV into Bombyx mori cells.
  • To identify potential therapeutic targets for BmCPV infections.

Main Methods:

  • Electron microscopy was employed to visualize BmCPV entry into BmN cells.
  • Endocytosis inhibitors (dansylcadaverine, chlorpromazine, genistein, PP2) were used to block entry pathways.
  • RNA interference and antibody-mediated functional blockade were used to target clathrin and AP-1.
  • Viral infectivity was assessed following inhibition of specific cellular processes.

Main Results:

  • Electron microscopy confirmed that BmCPV enters BmN cells through endocytosis.
  • Inhibitors of endocytosis significantly reduced BmCPV infectivity, indicating its dependence on this process.
  • Clathrin-mediated endocytosis was identified as the predominant entry mechanism.
  • Inhibition of clathrin heavy chain and AP-1 expression or function markedly decreased BmCPV infectivity.

Conclusions:

  • BmCPV utilizes endocytosis, specifically clathrin-mediated endocytosis, for cellular entry into BmN cells.
  • The clathrin-mediated endocytosis pathway represents a promising target for developing novel therapeutics against silkworm cytoplasmic polyhedrosis.

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