Hsa_Circ_0001275: A Potential Novel Diagnostic Biomarker for Postmenopausal Osteoporosis
Kewei Zhao1,2, Qing Zhao2, Zhaodi Guo3
1Nanfang Hospital, Southern Medical University, Guangzhou, China.
Insights
Researchers identified hsa_circ_0001275 as a potential diagnostic biomarker for postmenopausal osteoporosis (PMOP). This circular RNA (circRNA) shows diagnostic value in peripheral blood mononuclear cells (PBMCs) for PMOP detection.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) are emerging as promising diagnostic biomarkers.
- Postmenopausal osteoporosis (PMOP) is a significant health concern requiring reliable diagnostic tools.
- Peripheral blood mononuclear cells (PBMCs) offer a non-invasive source for biomarker discovery.
Purpose of the Study:
- To identify a potential diagnostic biomarker for PMOP from PBMCs.
- To investigate the expression and diagnostic value of circRNAs in PMOP patients.
Main Methods:
- CircRNA expression profiling using microarray and quantitative reverse transcription polymerase chain reaction (qRT-PCR).
- Validation in a cohort of PMOP patients and age- and sex-matched controls.
- Correlation analysis with clinical variables and receiver operator characteristic (ROC) curve analysis for diagnostic accuracy.
Main Results:
- Six differentially expressed circRNAs were detected, with hsa_circ_0001275 showing consistent and statistically significant expression.
- hsa_circ_0001275 was negatively correlated with T-score, a key indicator of bone density.
- ROC curve analysis indicated significant diagnostic value for hsa_circ_0001275 in PMOP (AUC=0.759).
Conclusions:
- hsa_circ_0001275 demonstrates potential as a novel diagnostic biomarker for PMOP.
- Further validation is warranted to establish its clinical utility in diagnosing PMOP.
Background/Aims:
Circular RNAs (circRNAs) serve as potential diagnostic biomarkers. In this study, we aimed to identify a potential biomarker from peripheral blood mononuclear cells (PBMCs) of patients with postmenopausal osteoporosis (PMOP).
Methods:
CircRNA expression in PBMCs from three pairs of samples from PMOP patients and controls was initially detected by circRNA microarray. The changes in selected circRNAs in PBMCs from 28 PMOP patients and 21 age- and sex-matched controls were confirmed using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Next, samples from 30 PMOP patients and 20 controls were used for further verification. Pearson correlation test was performed to assess the correlation between circRNAs and clinical variables. The area under the receiver operator characteristic (ROC) curve was calculated to evaluate the diagnostic value.
Results:
Six differentially expressed circRNAs were identified by chip microarray analysis, of which only hsa_circ_0001275 showed consistency and statistical significance in qRT-PCR. The correlation analysis between age, body weight, height, WBC, lymphocyte and monocyte count, bone density, T-score, β-CROSSL, OSTEOC, and TP1NP showed that hsa_circ_0001275 was negatively correlated with T-score. ROC curves showed that hsa_circ_0001275 has significant diagnostic value in PMOP (AUC=0.759, P< 0.001).
Conclusion:
This study suggests that hsa_circ_0001275 may serve as a potential diagnostic biomarker for PMOP.
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