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Updated: Feb 10, 2026

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Published on: October 5, 2016
Comparative metabolomics and glycolysis enzyme profiling of embryogenic and nonembryogenic grape cells
Jonathan Parrilla1, Cécile Gaillard1, Jérémy Verbeke1,2
1Laboratoire EBI- Ecologie et Biologie des Interactions Équipe SEVE-Sucres et Échanges Végétaux-Environnement UMR 7267 Centre National de la Recherche Scientifique Université de Poitiers France.
Abstract:
A novel biological model was created for the comparison of grapevine embryogenic cells (EC) and nonembryogenic cells (NEC) sharing a common genetic background but distinct phenotypes, when cultured on their respective most appropriate media. Cytological characterization, 1H-NMR analysis of intracellular metabolites, and glycolytic enzyme activities provided evidence for the marked metabolic differences between EC and NEC. The EC were characterized by a moderate and organized cell proliferation, coupled with a low flux through glycolysis, high capacity of phosphoenolpyruvate carboxylase and glucokinase, and high oxygen consumption. The NEC displayed strong anarchic growth, and their high rate of glycolysis due to the low energetic efficiency of the fermentative metabolism is confirmed by increased enolase capacity and low oxygen consumption.
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