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Updated: Feb 10, 2026

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Experimental and Computational Protocols for Studies of Cross-Seeding Amyloid Assemblies
Baiping Ren1, Rundong Hu1, Mingzhen Zhang1
1Department of Chemical & Biomolecular Engineering, The University of Akron, Akron, OH, USA.
Abstract:
Alzheimer's disease (AD) and type 2 diabetes (T2D) are two common protein aggregation diseases. Compelling evidence has shown a link between AD and T2D, which may derive from interspecies cross-sequence interactions between amyloid-β peptide (Aβ), associated with AD, and human islet amyloid polypeptide (hIAPP), associated with T2D. Herein, we present experimental and computational protocols and tools to study the aggregate structures and kinetics, conformational conversion, and molecular interactions of Aβ-hIAPP mixtures. These protocols could be generally applied to other cross-seeding behaviors of amyloid peptides.
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