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Inhibition of late influenza virus genome expression by diamidinophenylindole
G Conti1, P Portincasa, C Chezzi
1Instituto di Microbiologia, Facoltà di Medicina e Chirurgia, Università delgi Studi, Parma, Italy.
Abstract:
The growth cycle of influenza virus strain FPV, Ulster 73, was altered by treatment of LLC-MK2 cells with diamidinophenylindole. Viral protein synthesis was restricted to the early pattern of virus multiplication, and post-treatment experiments showed the ability of the drug to block virus replication until the 4th hour p.i. Drug addition (followed by removal) revealed the inhibition of synthesis of late viral products, and especially of membrane protein. Kinetic studies on the production of viral RNA indicated a decrease in the synthesis of late virus-induced RNA species, suggesting that the target of DAPI is probably the late transcription of the virus genome. The nonpermissive condition mediated by the drug could represent a suitable model to study cellular intervention during viral growth.
Insights
Diamidinophenylindole (DAPI) treatment alters influenza virus replication in LLC-MK2 cells, restricting viral protein synthesis and blocking replication. DAPI likely targets late viral genome transcription, offering a model for studying cellular intervention in virus growth.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Influenza virus replication is a complex process involving intricate interactions between viral and cellular machinery.
- Understanding the molecular mechanisms that regulate viral gene expression is crucial for developing antiviral strategies.
Purpose of the Study:
- To investigate the effects of diamidinophenylindole (DAPI) on the replication cycle of influenza virus strain FPV, Ulster 73.
- To elucidate the specific stage of viral replication targeted by DAPI and its impact on viral gene expression.
Main Methods:
- Treatment of LLC-MK2 cells with DAPI during influenza virus infection.
- Analysis of viral protein synthesis patterns.
- Kinetic studies on viral RNA production.
- Assessment of viral replication progression post-treatment.
Main Results:
- DAPI treatment restricted viral protein synthesis to an early pattern.
- The drug blocked virus replication until 4 hours post-infection.
- Inhibition of late viral product synthesis, particularly membrane proteins, was observed.
- DAPI reduced the synthesis of late virus-induced RNA species, suggesting a target in late transcription.
Conclusions:
- Diamidinophenylindole (DAPI) interferes with late-stage influenza virus transcription and replication.
- The nonpermissive state induced by DAPI provides a model for studying cellular involvement in viral growth.