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Updated: Feb 10, 2026

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
Published on: February 10, 2023
A simple and rapid LC-MS/MS method for the determination of amphenicols in Nile tilapia
Letícia Rocha Guidi1, Patrícia Amaral Souza Tette1, Maria Beatriz A Gloria1
1LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, Belo Horizonte, MG 31270-901, Brazil.
Abstract:
A quantitative method for analysis of amphenicols (chloramphenicol - CAP, thiamphenicol - TAP and florfenicol - FF) in Nile tilapia using LC-MS/MS is described. A simple sample preparation procedure was optimized using a Plackett-Burman design. The method was validated in accordance with Decision 2002/657/EC. Repeatability and reproducibility were less than 10.7% and 16%, respectively, for all compounds. Recoveries varied from 79.8% to 92.0%. CCα was 0.019, 54.81 and 54.93 μg.kg-1 for CAP, FF and TAP, respectively. CCβ was 0.068, 64.88 and 58.91 μg.kg-1 for CAP, FF and TAP, respectively. Limits of quantification (LOQ) were 12.5 μg.kg-1 for FF and TAP and 0.15 μg.kg-1 for CAP. Nile tilapia fillets (n = 32) analyzed did not contain chloramphenicol. Thiamphenicol was detected in one sample (3.1%) and florfenicol was detected in every sample, all of them at concentrations below the maximum residue limit.
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