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Engineering synergetic CO2-fixing pathways for malate production.

Guipeng Hu1, Jie Zhou2, Xiulai Chen1

  • 1State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.

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|May 14, 2018
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Summary
This summary is machine-generated.

This study introduces a novel strategy to enhance microbial carbon dioxide (CO2) fixation by combining ATP generation with a CO2-fixing pathway. This approach boosts CO2 fixation rates and metabolite production in both autotrophic and heterotrophic microbes.

Keywords:
ATP balanceCO(2) fixationMalate productionPathway engineering

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Area of Science:

  • Synthetic biology
  • Metabolic engineering
  • Microbial biotechnology

Background:

  • Microbial CO2 fixation requires sufficient ATP and redirected carbon flux for metabolite production.
  • Simultaneously addressing ATP supply and carbon flux redirection for enhanced CO2 fixation is challenging.

Purpose of the Study:

  • To develop a combinational strategy for enhancing microbial CO2 fixation efficiency.
  • To concurrently supply ATP and rewire carbon flux for improved metabolite biosynthesis.

Main Methods:

  • Integration of an ATP-generating carboxylation reaction into the central metabolic pathway.
  • Incorporation of an ATP-consuming RuBisCO shunt into the carbon fixation pathway.
  • Application of the synergetic CO2-fixing pathway in autotrophic (Synechococcus elongatus) and heterotrophic (Escherichia coli) microbes.

Main Results:

  • Demonstrated increased CO2-fixing rates and malate production in Synechococcus elongatus by 110% and to 260 μM, respectively.
  • Achieved an 870% increase in CO2-fixing rate and 387 mM malate production in Escherichia coli.
  • Successfully coupled ATP generation with CO2 fixation for enhanced carbon flux towards chemical biosynthesis.

Conclusions:

  • The developed synergetic CO2-fixing pathway effectively enhances microbial carbon fixation and metabolite production.
  • This strategy offers a promising approach for engineering microbes for efficient carbon capture and chemical synthesis.
  • The combined metabolic engineering approach provides a robust platform for optimizing microbial CO2 utilization.