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Updated: Feb 10, 2026

Recombineering Homologous Recombination Constructs in Drosophila
Published on: July 13, 2013
Novel Vector Construction Based on Alternative Adenovirus Types via Homologous Recombination
Wenli Zhang1, Jun Fu2, Anja Ehrhardt1
11 Chair for Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department of Human Medicine, Faculty of Health, Witten/Herdecke University , Witten, Germany .
Abstract:
Adenoviral vector (AdV) is one of the most used vectors in gene therapy clinical trials. However the therapeutic effect of AdV is limited due to preexisting immunity to the currently used human adenovirus type 5 and pre-decided vector tropism. It is highly demanded to develop novel AdVs originated from other types than adenovirus type 5. Here, we describe a method for direct cloning of adenovirus utilizing linear-linear homologous recombination, followed by rapid adenoviral genome modification via linear-circular homologous recombination. A plasmid bearing chosen adenoviral genome with the desired modification is generated in three weeks, from which a novel AdV can be reconstituted.
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