Related Experiment Video
Updated: Feb 10, 2026

Using Flight Mills to Measure Flight Propensity and Performance of Western Corn Rootworm, Diabrotica virgifera virgifera LeConte
Published on: October 29, 2019
Development and characterization of the first dsRNA-resistant insect population from western corn rootworm,
Chitvan Khajuria1, Sergey Ivashuta1, Elizabeth Wiggins1
1Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.
Abstract:
The use of dsRNA to control insect pests via the RNA interference (RNAi) pathway is being explored by researchers globally. However, with every new class of insect control compounds, the evolution of insect resistance needs to be considered, and understanding resistance mechanisms is essential in designing durable technologies and effective resistance management strategies. To gain insight into insect resistance to dsRNA, a field screen with subsequent laboratory selection was used to establish a population of DvSnf7 dsRNA-resistant western corn rootworm, Diabrotica virgifera virgifera, a major maize insect pest. WCR resistant to ingested DvSnf7 dsRNA had impaired luminal uptake and resistance was not DvSnf7 dsRNA-specific, as indicated by cross resistance to all other dsRNAs tested. No resistance to the Bacillus thuringiensis Cry3Bb1 protein was observed. DvSnf7 dsRNA resistance was inherited recessively, located on a single locus, and autosomal. Together these findings will provide insights for dsRNA deployment for insect pest control.
More Related Videos
07:42Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
Published on: April 27, 2018
06:52Characterizing Herbivore Resistance Mechanisms: Spittlebugs on Brachiaria spp. as an Example
Published on: June 19, 2011
Related Concept Videos
Development of Antibiotic Resistance
Osmoregulation in Insects
Conservation of Small Populations
What is Population Genetics?
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
Population Growth