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In vitro effect of PTH on normal T cell functions
S M Shasha1, B Kristal, M Barzilai
1Eliachar Research Laboratory, Western Galilee Regional Hospital, Nahariya, Israel.
Nephron
|January 1, 1988
Summary
Elevated parathyroid hormone (PTH) in uremia impairs T cell functions, including lymphocyte transformation and helper-to-suppressor ratios. This suggests PTH contributes to immune dysfunction in kidney disease.
Area of Science:
- Immunology
- Nephrology
- Endocrinology
Background:
- Uremia is linked to impaired cell-mediated immunity.
- Parathyroid hormone (PTH) levels are elevated in uremia.
Purpose of the Study:
- To investigate the specific effects of parathyroid hormone (PTH) on T cell functions in normal lymphocytes.
- To determine if elevated PTH contributes to immune impairment in uremia.
Main Methods:
- Incubation of normal peripheral blood lymphocytes with varying concentrations of human PTH (HPTH) and bovine PTH (BPTH).
- Assessment of lymphocyte transformation, helper to suppressor cell ratios, E rosette formation, and T11-positive cells.
- Control experiments with glucagon to assess specificity of PTH effects.
Main Results:
- PTH significantly decreased lectin-induced lymphocyte transformation by up to 40%.
- PTH caused a significant reduction in the helper to suppressor cell ratio.
- Marked inhibition of E rosette formation and T11-positive cells was observed with PTH exposure.
- Glucagon did not affect T cell function, indicating PTH's specific action.
Conclusions:
- Excess parathyroid hormone (PTH) directly affects normal T lymphocytes and their functions.
- Elevated PTH levels may play a significant role in the immune response impairment observed in uremia.
- The findings support the hypothesis linking high PTH to uremic immune dysfunction.