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Increase in myoglobin content and decrease in oxidative enzyme activities by leg muscle immobilization in man.

E Jansson1, C Sylvén, I Arvidsson

  • 1Department of Clinical Physiology, Karolinska Hospital, Stockholm, Sweden.

Acta Physiologica Scandinavica
|April 1, 1988
PubMed
Summary
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Postoperative knee surgery immobilization reduced oxidative enzymes like citrate synthase and creatine kinase in thigh muscles. However, myoglobin content increased, potentially enhancing oxygen extraction.

Area of Science:

  • Muscle physiology
  • Biochemistry
  • Orthopedic surgery

Background:

  • Immobilization following knee surgery can lead to muscle atrophy and altered metabolic profiles.
  • Understanding the impact of immobilization on specific muscle enzymes and proteins is crucial for rehabilitation strategies.

Purpose of the Study:

  • To investigate the effects of postoperative knee immobilization on various muscle biochemical markers.
  • To determine changes in myoglobin and key metabolic enzymes in the quadriceps femoris muscle.

Main Methods:

  • Muscle biopsies were obtained from the quadriceps femoris of nine patients before and 6 weeks after knee surgery.
  • Analysis included myoglobin content and activities of citrate synthase, creatine kinase (and its isozymes), aspartate aminotransferase, phosphofructokinase, and lactate dehydrogenase.

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Main Results:

  • Activities of citrate synthase, aspartate aminotransferase, creatine kinase (total, MB, and mitochondrial), and lactate dehydrogenase decreased by 12-30% after immobilization.
  • Phosphofructokinase activity remained unchanged.
  • Myoglobin content increased by 16% following the immobilization period.

Conclusions:

  • Postoperative immobilization differentially affects muscle protein synthesis, decreasing oxidative enzyme activity while increasing myoglobin.
  • The observed increase in myoglobin may play a role in facilitating oxygen extraction during recovery.
  • These findings suggest distinct regulatory mechanisms for oxidative enzymes and myoglobin in response to immobilization.