Related Experiment Video

Updated: Feb 10, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

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MiRNA-93 functions as an oncogene in glioma by directly targeting RBL2

D-K Liu1, Y-J Wei, Y Guo

  • 1School of Clinical Medicine, Tsinghua University, China. neurospinewgh@163.com.

European Review for Medical and Pharmacological Sciences
|May 16, 2018
PubMed
Abstract

Insights

MicroRNA-93 (miR-93) promotes glioma cell invasion and migration. Targeting miR-93 and its downstream target RBL2 may offer new diagnostic and prognostic strategies for glioma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Glioma therapies face challenges, particularly in advanced stages.
  • MicroRNAs (miRNAs) are implicated in glioma development.
  • miR-93 is a novel, potentially cancer-promoting miRNA in glioma.

Purpose of the Study:

  • Investigate the function of miR-93 in glioma progression.
  • Determine the expression and mechanism of miR-93 in glioma.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) for gene expression analysis.
  • Transwell assays to assess cell invasion and migration.
  • Luciferase reporter assay to confirm miRNA targets.

Main Results:

  • miR-93 expression is significantly elevated in glioma tissues and cells.
  • miR-93 enhances glioma cell migration and invasion.
  • RBL2 is a direct target of miR-93; its overexpression reverses miR-93's effects.

Conclusions:

  • miR-93 promotes glioma progression by targeting RBL2.
  • miR-93 and RBL2 show potential as diagnostic and prognostic markers for glioma.

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